blomia tropicalis wikipedia
Since A/J mice had more intense allergic responses, we selected this strain to make a comparison between the BtE-induced asthma model with the classical OVA-induced asthma model. (F) IL-13 concentration in BALF. The effect of BtE and OVA sensitizations on total IgE and specific antibodies levels are shown in Figure 4. Am J Respir Cell Mol Biol 1999, 21:480–89. tropicalis allergic responses in different mouse strains using a short-term model of respiratory allergy to BtE. tropicalis IgE antibody levels as titrated by passive cutaneous anaphilaxis (PCA). Total IgE was higher in BtE-sensitized animals (Figure 4A; p < 0001) and specific-IgE, IgG2a and IgG1 antibodies were higher in OVA-sensitized group (Figure 4B-D; p < 0.05, Tukey's test). Following centrifugation (400 g, 5 min, 4°C), supernatants of the BALF were collected and stored at -70°C for subsequent measurement of cytokine content and the pellets were used for the measurement of eosinophil peroxidase (EPO) activity. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The described short-term model of BtE-induced allergic lung disease is reproducible in different syngeneic mouse strains, and mice of the A/J strain was the most responsive to it. J Biol Chem 1951, 193:265–275. However, in humans, most cases of asthma are due to aeroallergens, and OVA-induced asthma is far from being a common event. Its use as an assay for eosinophil differentiation factors. Altogether, we concluded that the short-term experimental model of BtE-induced asthma is reproducible in different mouse strains, although the A/J mice are the best responders, and small quantities of BtE may be used to sensitize this mouse strain. This article is published under license to BioMed Central Ltd. This work was supported by the Brazilian Ministry of Science and Technology (RENORBIO programme and Conselho Nacional de Pesquisa e Desenvolvimento Tecnológico - CNPq), the Fundações de Amparo à Pesquisa dos Estados da Bahia e São Paulo (FAPESB and FAPESP), and the Wellcome Trust (Grant No. Hum Genet 2004,115(4):295–301. This data is representative of three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001. The effect of sensitization and challenge with 10 μg of BtE per injection on lung histology is seen in Figures 5F and 5G. Erb KJ: Helminths, allergic disorders and IgE-mediated immune responses: Where do we stand? *p < 0.05, **p < 0.01, and ***p < 0.001 for the indicated tested groups (Tukey's test). Finally, we used a low dose of BtE (10 μg/per subcutaneous injection) and obtained results that were similar to those obtained with a high dose (100 μg/per mouse) protocol, showing that BtE is able to sensitize A/J mice even at small concentrations. Serum anti-OVA or anti-BtE IgG1 and IgG2a antibodies were measured using OVA- or BtE-coated microtitre plates and biotin-conjugated anti-mouse IgG1 or anti-mouse IgG2a, respectively (Pharmingen, St. Diego, CA, USA), in conjunction with streptavidin-horseradish peroxidase, H2O2 and orthophenylenodiamine (Merck, Whitehouse Station, NJ, USA). El este așa de mic, încât poate fi transportat de particulele de praf ce plutesc în aer sau de mici curenți de aer. Although a mouse model for B. tropicalis extract (BtE)-induced asthma has been described, no study comparing different mouse strains in this asthma model has been reported. Blomia tropicalis allergens are the most important mite allergens in tropical regions. Acta Paediatr Taiwan 2006,47(3):127–34. After the BALF collection, the lungs were perfused, via the heart right ventricle, to remove residual blood, immersed in 10% phosphate-buffered formalin for 24 h, followed by 70% ethanol, and embedded in paraffin. Lack of human IgE cross … Article Rizzo MC, Fernandez-Caldas E, Sole D, Naspitz CK: IgE antibodies to aeroallergens in allergic children in Sao Paulo, Brazil. The amount BtE used in the experiments was standardized by measuring its content in B. tropicalis Blo t 5 allergen, measured by a commercially available capture ELISA kit (INDOOR Biotechnologies, Charlottesville, VI, USA). This study included the comparison of the allergic responses elicited by BtE with those elicited by ovalbumin in mice of the strain that responded better to BtE sensitization. JCI 2006,116(2):306–8. (C) Level of eosinophil peroxidase (EPO) activity in BALF. No differences in numbers of macrophage, lymphocyte and neutrophils in the BALF were observed among the mice of all four strains (p > 0.05, ANOVA; data not shown). Effect of immunization with 10 μg per injection ofB. Mice of some strains develop an intense airway hyperreactivity, eosinophilia and IgE production, while others fail to produce allergic responses . However, results obtained in murine experimental models of respiratory allergy that use OVA as antigen differ from those obtained in experimental models using mite allergens. (D) Anti-B. Despite the bulk of work done in humans on mite-specific allergy, data on allergic responses to B. tropicalis antigens in murine models are scarce [14–16]. (A) Total leukocyte numbers in the bronchoalveolar lavage fluid (BALF). An aliquot of the BALF cells was washed three times by centrifugation, and the cell pellet resuspended in PBS-BSA. PubMed Neuza M Alcântara-Neves. https://doi.org/10.1186/1465-9921-11-51, DOI: https://doi.org/10.1186/1465-9921-11-51. Accordingly, sensitization of A/J mice to BtE led to pulmonary inflammation with eosinophil infiltrate and to total IgE increase, while OVA sensitization produced low eosinophil and IL-4 responses in this mouse strain. Although a mouse model for B. tropicalis extract (Bt E)-induced asthma has been described, no study comparing different mouse strains in this asthma model has been reported. EG and RB helped in the experiments with OVA- and BtE-induced asthma models. P > 0.05 is not represented. OVA-sensitized mice also showed increased total cell (p < 0.05; Figure 2A; Tukey's test) and eosinophil counts (p < 0.05; Figure 2B; Tukey's test) in the BALF than the corresponding control, saline-treated animals. Figure 1G shows that specific IgG1 was produced in all BtE-sensitized mice and that its levels were statistically different from those of the control mice (p < 0.001 for BALB/C, C57Bl/6, and A/J mice, and p < 0.05 for CBA/J; Tukey's test). The mites were purified from the medium by flotation on a 5 M sodium chloride solution, followed by several washings by filtration, using a 100 μm pore size polystyrene sieve and endotoxin-free distilled water. A/J mice, that were the best responders to BtE sensitization, were used to compare the B. tropicalis-specific asthma experimental model with the conventional experimental model of ovalbumin (OVA)-specific asthma. The allergenity of B. tropicalis antigens to mice has been demonstrated before [14–16], although without a detailed investigation using different strains of mice and different doses of antigen. NMANeves was T B's post-graduation adviser, planned the experiments, and reviewed the manuscript. We sought to compare allergen inflammatory pulmonary response in previously sensitized mice orally administered with dust mites Blomia tropicalis (Bt) or Dermatophagoides pteronyssinus (Dp). LPC participated in planning the experiments and reviewing the manuscript. The following parameters were used to measure the immune response in mice of four inbred strains (CBA/J, BALB/c, A/J and C57Bl/6): (i) the total number of leukocytes and eosinophils in the bronchoalveolar lavage fluid (BALF); (ii) the concentration of IL-4 and IL-13 cytokines and eosinophil peroxidase (EPO) in the BALF; (iii) the serum levels of anti-B. Statistically significant when p ≤ 0.05 dust mite allergens [ 24 ] in four syngeneic strains. Allergens are the most potent allergens in house dust un fundal întunecat la o lumină normală the experiments different! [ 23 ], TM and PRSO helped in the sera ( d201 ) IgE anti-Bt antibody serum titers determined... Being reported in experimental models of asthma are due to aeroallergens in allergic in! * * p < 0.05, * * p < 0.05, *. Epo activity present in BALF was determined using the Komogorov-Smirnov test Vogel J: experimental model, domesticus. 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